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deepvent  (New England Biolabs)


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    Structured Review

    New England Biolabs deepvent
    Deepvent, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deep+vent+polymerase/Deep+Vent+DNA+Polymerase/pmc13601594-293-6-17
    Average 96 stars, based on 3335 article reviews
    deepvent - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A new generation of nanobody research tools using improved mass spectrometry-based discovery methods
    Article Snippet: RNA was isolated using TRIzol (Thermo Fisher Scientific), and cDNA was generated using SuperScript IV reverse transcriptase with oligo(dT) primers (Thermo Fisher Scientific). .. V H H domains were then PCR amplified using Deep Vent polymerase (NEB). ..

    Article Title: A new generation of nanobody research tools using improved mass spectrometry-based discovery methods.
    Article Snippet: Single domain antibodies (“nanobodies”) derived from the variable region of camelid heavy-chain only antibody variants have proven to be widely useful tools for research, as well as therapeutic and diagnostic applications.. In addition to traditional display techniques, methods to generate nanobodies using direct detection by mass spectrometry and DNA sequencing have been highly effective.. However, certain technical challenges have limited widespread application.

    Article Title: Chimeric primers with hairpin conformations and methods of using same
    Article Snippet: .. The following cocktail was placed in the 25 μL volume glass chamber: TABLE 15 10x ThermoPol Buffer 5 μl H2O 34.7 μl Primer 200 nM each 12SNAP/ptu & PtdCY3 Template #24 0.3 μl (100 ng) Beads 2 μl (5,000 beads) dNTPs 1 μl (0.2 mM each) Deep Vent Polymerase 2 μl (10 Units) (New England BioLabs) MgSO4 3 μl (8 mM) Total 50 μl The following PCR Cycling conditions were run: 1) 97° C. for 5 min; 2) 105° C. for 15s; 3) 96° C. for 30s; 4) 50° C. for 5s; 5) 68° C. for 30s; 6) Go to 2, 5 times; 7) 15° C. for 10s; 8) 24° C. for 5 min; 9) Go to 2, 6 times; 10) End. ..

    Article Title: Chimeric primers with hairpin conformations and methods of using same
    Article Snippet: .. The following cocktail was placed in the 25 μL volume glass chamber: TABLE 15 10x ThermoPol Buffer 5 μl H2O 34.7 μl Primer 200 nM each 12SNAP/ptu & PtdCY3 Template #24 0.3 μl (100 ng) Beads 2 μl (5,000 beads) dNTPs 1 μl (0.2 mM each) Deep Vent Polymerase 2 μl (10 Units) (New England BioLabs) MgSO4 3 μl (8 mM) Total 50 μl The following PCR Cycling conditions were run: 1) 97° C. for 5 min; 2) 105° C. for 15 s; 3) 96° C. for 30 s; 4) 50° C. for 5 s; 5) 68° C. for 30 s; 6) Go to 2, 5 times; 7) 15° C. for 10 s; 8) 24° C. for 5 min; 9) Go to 2, 6 times; 10) End. ..

    Article Title: Chimeric primers with hairpin conformations and methods of using same
    Article Snippet: .. TABLE 13 10x ThermoPol Buffer 5 μl H2O 34.7 μl Primer 2 μl (400 nM) Template #24 0.3 μl (100 ng) Beads 2 μl (5,000 beads) dNTPs 1 μl (0.2 mM) Deep Vent Polymerase 2 μl (10 Units) (New England BioLabs) MgSO4 3 μl (8 mM) Total 50 μl TABLE 14 Analyte Analyte Analyte Total Location Sample 27 MFI 29 MFI 33 Events 1(1, A1) 12SNAP/ptu, 14.5 10 23 184 no template 2(1, B1) 12SNAP/ptu 10.5 78 12.5 415 3(1, C1) 12SNAP/ptu 6.5 84 14 322 4(1, D1) PT TIF, 7 16 17 346 5(1, E1) PT TIF 15 38 14 336 6(1, F1) PT TIF 6 36.5 10.5 193 From the results in Table 14, it can be seen that the signal difference of the 12SNAP/ptu primer is about double that of the PT TIF primer after 27 cycles using 400 nM primer concentrations with a PCR protocol of: 97° C., 5 minutes; (97° C., 30 seconds; 55° C., 30 seconds; 72° C., 30 seconds)×27 cycles; followed by 7 minutes at 72° C. These results also demonstrated that the PT primers will not cross hybridize with the MHFTR primer sets if combined into a multiplex reaction. ..

    Amplification:

    Article Title: A new generation of nanobody research tools using improved mass spectrometry-based discovery methods
    Article Snippet: RNA was isolated using TRIzol (Thermo Fisher Scientific), and cDNA was generated using SuperScript IV reverse transcriptase with oligo(dT) primers (Thermo Fisher Scientific). .. V H H domains were then PCR amplified using Deep Vent polymerase (NEB). ..

    Article Title: A new generation of nanobody research tools using improved mass spectrometry-based discovery methods.
    Article Snippet: Single domain antibodies (“nanobodies”) derived from the variable region of camelid heavy-chain only antibody variants have proven to be widely useful tools for research, as well as therapeutic and diagnostic applications.. In addition to traditional display techniques, methods to generate nanobodies using direct detection by mass spectrometry and DNA sequencing have been highly effective.. However, certain technical challenges have limited widespread application.

    Article Title: A CD25-biased interleukin-2 for autoimmune therapy engineered via a semi-synthetic organism
    Article Snippet: .. Insert template oligos were amplified at 1 ng per 50 μL reaction using a mixture of OneTaq DNA Polymerase and Deep Vent Polymerase (NEB), 0.5 μM of each primer, 1x OneTaq buffer, 0.5x of SybrGreen, 3.0 mM MgSO4, 200 μM dNTPs, 100 μM of each unnatural nucleotide. .. Inserts were purified using the Zymo Clean and Concentrator Kit.

    Article Title: A CD25-biased interleukin-2 for autoimmune therapy engineered via a semi-synthetic organism.
    Article Snippet: .. Insert template oligos were amplified at 1 ng per 50 µL reaction using a mixture of OneTaq DNA Polymerase and Deep Vent Polymerase (NEB), 0.5 µM of each primer, 1x OneTaq buffer, 0.5x of SybrGreen, 3.0mM MgSO4, 200 µM dNTPs, 100 µM of each unnatural nucleotide. .. Inserts were purified using the Zymo Clean and Concentrator Kit.

    Article Title: A CD25-biased interleukin-2 for autoimmune therapy engineered via a semi-synthetic organism.
    Article Snippet: .. Insert template oligos were amplified at 1 ng per 50 μL reaction using a mixture of OneTaq DNA Polymerase and Deep Vent Polymerase (NEB), 0.5 μM of each primer, 1x OneTaq buffer, 0.5x of SybrGreen, 3.0mM MgSO4, 200 μM dNTPs, 100 μM of each unnatural nucleotide. .. Inserts were purified using the Zymo Clean and Concentrator Kit.

    Multiplex Assay:

    Article Title: Chimeric primers with hairpin conformations and methods of using same
    Article Snippet: .. TABLE 13 10x ThermoPol Buffer 5 μl H2O 34.7 μl Primer 2 μl (400 nM) Template #24 0.3 μl (100 ng) Beads 2 μl (5,000 beads) dNTPs 1 μl (0.2 mM) Deep Vent Polymerase 2 μl (10 Units) (New England BioLabs) MgSO4 3 μl (8 mM) Total 50 μl TABLE 14 Analyte Analyte Analyte Total Location Sample 27 MFI 29 MFI 33 Events 1(1, A1) 12SNAP/ptu, 14.5 10 23 184 no template 2(1, B1) 12SNAP/ptu 10.5 78 12.5 415 3(1, C1) 12SNAP/ptu 6.5 84 14 322 4(1, D1) PT TIF, 7 16 17 346 5(1, E1) PT TIF 15 38 14 336 6(1, F1) PT TIF 6 36.5 10.5 193 From the results in Table 14, it can be seen that the signal difference of the 12SNAP/ptu primer is about double that of the PT TIF primer after 27 cycles using 400 nM primer concentrations with a PCR protocol of: 97° C., 5 minutes; (97° C., 30 seconds; 55° C., 30 seconds; 72° C., 30 seconds)×27 cycles; followed by 7 minutes at 72° C. These results also demonstrated that the PT primers will not cross hybridize with the MHFTR primer sets if combined into a multiplex reaction. ..



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